Core - NMR

Overview of Services

The DFCI NMR core offers walk-up NMR spectroscopy capabilities for samples of moderate concentration and complexity. This is a user-based NMR facility; users generally run their own experiments on the NMR spectrometer, with staff available to offer instruction and guidance on the best experiments for any particular job.

 

Nuclear magnetic resonance spectroscopy is a powerful tool for studying structural and conformational parameters of molecules, and is a flexible technique for measuring binding of ligands to cognate macromolecules. This tool is vital for the chemical analysis of organic compounds, compounds discovered in small-molecule screens, and large molecules, such as carbohydrates and proteins found to have potential biologic importance.

Equipment

500 MHz Bruker Avance III two-channel console, equipped with a temperature controlled SampleJet automation system. Acquisition capabilities include walk-up proton, fluorine, and carbon NMR, routine 1D and 2D experiments, variable temperature. The SampleJet has concurrent capacity for 30 traditional chemistry samples plus 5 x 96 samples that can be stored at low temperature (typically protein samples). The system uses a standard room temperature NMR probe which gives good proton and carbon sensitivity; fluorine sensitivity on this instrument will be the highest of any instrument in the NMR Core.

 

This is a walkup machine which typically operates in full automation mode. Special experiments (ie longer time) need to be cleared with Walt at least a week in advance.

Leadership

Greg Heffron, Core Director

gregory_heffron@hms.harvard.edu

617.432.1812

 

John Alberta, DFCI NMR Core Lab Interim Contact
john_alberta@dfci.harvard.edu

617.632.1987

Location and hours of operation

Hours Location

Available 24 hours, 7 days/week for trained users 

Longwood Center, 3rd floor, Room LC-3301

Links and Resources

See https://eqnmr.med.harvard.edu for a full catalog of NMR Core services and additional contact information. To schedule manual acquisition time, please use https://calendars.med.harvard.edu/nmr500 and send a note to Walt.

Contacts

Name Role Phone Email Location
John Alberta, PhD
Interim Contact
 
617.632.1987
 
john_alberta@dfci.harvard.edu
 
LC
 

Services

Name Description