ALERT

In observance of US Thanksgiving holiday, Agilent Cross Lab iLab Operations Software will be closed during US hours on Thursday November 23rd and Friday November 24th, 2017. EU and APAC support coverage will remain open during this time. We will resume US support hours on Monday November 27th, 2017. For urgent matters, please add "URGENT" to the ticket subject and these requests will be prioritized first.

Core - MBCF: Microarray

Overview of Services

The Microarray Core Facility offers Gene Expression Profiling, SNP, miRNA, and CNV analysis using Affymetrix microarray technologies.

The Core specializes in handling and processing RNA extracted from Formalin-Fixed Parafin embedded tissue (FFPE). 

 

Contact Us with questions or to discuss your project

Sample Drop off

Location – "Fish Room" near 3rd floor elevator lobby of the Smith Building. Next to the multicolored fish sculpture.

Entry – 
If you are a DFCI employee, use your DFCI ID card to enter the room.

If you are not a DFCI employee, first obtain a Visitor Pass at the Security Desk in the Smith Building lobby, then dial 2-3131 on the red phone near SM300, and ask for SM300 to be unlocked.

 

Lab location and hours of operation

Hours Location

Monday-Friday               

21-27 Burlington Ave. 4th floor

 

Information for Users External to DFCI

Purchase Orders are to be made out to:

Dana Farber Cancer Institute

General Accounting, BP-425

450 Brookline Avenue

Boston, MA 02215-5450

 

Note to Partners, Harvard, Boston Children’s and Tufts researchers:

  • Partners Health Care (includes MGH, BWH, McLean, Spaulding, etc): choose vendor 0000008573
  • Harvard (includes HMS,Dental, Wyss, HSPH, etc.): choose vendor site: 01_PRIMARY
  • Tufts University: choose Supplier 0000000655
  • Boston Children’s Hospital: choose vendor 0000001886

 

 If you are using a PO from an institution other than one under which you signed in, you must stop and contact the DFCI iLab staff at sc_billing@dfci.harvard.edu


 

Links and Resources

http://macf-web.dfci.harvard.edu/

Contacts

Name Role Phone Email Location
Zach Herbert
Associate Director Genomics Core Facility
 
617-632-3082
 
zherbert@mail.dfci.harvard.edu
 

 
Maura Berkeley
Lab Supervisor
 

 
maura_berkeley@dfci.harvard.edu
 

 
Paul Morrison
Director
 
617-632-3082
 
paul_morrison@dfci.harvard.edu
 

 

Services

Name Description
Custom processing   
DNA - Bioanalyzer 12000 

 

x ng/uL, submit 5 uL

 
DNA Bioanalyzer - High Sensitivity 


x ng/uL, submit 5 uL

 
Drosophila Genome 2.0 

 

Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 
Drosophila Genome 2.0 

 

Sample Preparation: NuGEN Ovation V2

Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

More information: Datasheet

 

 
Genome-Wide Human SNP 6.0 

Sample requirement: 600ng* genomic DNA supplied at 50ng/uL

 

(*minimum 500ng supplied at 50ng/uL)

 

Datasheet

 

 

 
Human Cytoscan HD 

Sample requirement: 400ng* genomic DNA supplied at 50ng/uL

 

(*minimum 250ng supplied at 50ng/uL)

 

More information: Datasheet

 
Human Exon 1.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

More information: Datasheet

 

 
Human Exon 1.0 ST 

 

Affy Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More Information: Datasheet

 
Human Exon 1.0 ST 

 

Sample requirement: less than 50ng FFPE total RNA

 

Datasheet

 
Human Exon 1.0 ST 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 
Human Gene 1.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

More information: Datasheet

 
Human Gene 1.0 ST 

 

NuGEN Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More Information: Datasheet

 
Human Gene 1.0 ST 

 

Sample requirement: less than 50ng FFPE total RNA

 

Datasheet

 
Human Gene 1.0 ST 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 
Human Gene 2.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

More information: Datasheet

 

 
Human Gene 2.0 ST 

 

NuGEN Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More Information: Datasheet

 

 
Human Gene 2.0 ST 

 

Sample requirement: less than 50ng FFPE total RNA

 

Datasheet

 

 
Human Gene 2.0 ST 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 
Human Genome U133A 2.0 

 

Sample Preparation: NuGEN Ovation V2

Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

More information: Datasheet

 

 
Human Genome U133A 2.0 


Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 

 

 
Human Genome U133Plus 2.0 


Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 

 
Human Genome U133Plus 2.0 

 

Sample Preparation: NuGEN Ovation V2

Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

More information: Datasheet

 

 
Human Mapping 250K - Nsp 

Sample requirement: 400ng* genomic DNA supplied at 50ng/uL

 

(*minimum 250ng supplied at 50ng/uL)

 

More information: Datasheet

 

 
Human Transcriptome Array (HTA) 2.0 

 

Sample requirement: 300ng (minimum 100ng)

 

More information:Datasheet

 

 
Human Transcriptome Array (HTA) 2.0 

 

NuGEN Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More Information: Datasheet

 
Human Transcriptome Array (HTA) 2.0 

 

Sample requirement: less than 50ng FFPE total RNA

 

Datasheet

 
Human Transcriptome Array (HTA) 2.0 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 
miRNA 4.0 Array   
Mouse Diversity Genotyping 

Sample requirement: 600ng* genomic DNA supplied at 50ng/uL

 

(*minimum 500ng supplied at 50ng/uL)

 

More Information: Datasheet

 
Mouse Exon 1.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

Datasheet

 

 
Mouse Exon 1.0 ST 

 

NuGEN Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More Information: Datasheet

 
Mouse Exon 1.0 ST 

 

Sample requirement: less than 50ng FFPE total RNA

 

Datasheet

 
Mouse Exon 1.0 ST 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 
Mouse Gene 1.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

More information: Datasheet

 

 
Mouse Gene 1.0 ST 

 

NuGEN Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More Information: Datasheet

 
Mouse Gene 1.0 ST 

 

Sample requirement: less than 50ng FFPE total RNA

 

Datasheet

 
Mouse Gene 1.0 ST 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 
Mouse Gene 2.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

More information: Datasheet

 

 
Mouse Gene 2.0 ST 

 

NuGEN Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More Information: Datasheet

 
Mouse Gene 2.0 ST 

 

Sample requirement: less than 50ng FFPE total RNA

 

Datasheet

 
Mouse Gene 2.0 ST 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 
Mouse Genome 430 2.0 


Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 
Mouse Genome 430 2.0 

 

Sample Preparation: NuGEN Ovation V2 

Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

More information: Datasheet

 

 
Mouse Genome 430A 2.0 

 

Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 
Mouse Genome 430A 2.0 

 

Sample Preparation: NuGEN Ovation V2 

Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

More information: Datasheet

 
Mouse Transcriptome Array   
PrimeView 

 

Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 

 

 
PrimeView 

 

Sample Preparation: NuGEN Ovation V2 

Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

More information: Datasheet

 

 
Rat Gene 1.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

More information: Datasheet

 

 
Rat Gene 1.0 ST 

 

NuGEN Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More information: Datasheet

 
Rat Gene 1.0 ST 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 
Rat Gene 2.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

More information: Datasheet

 

 
Rat Gene 2.0 ST 

 

NuGEN Pico WTA

 

Sample Requirement: Less than 50ng total RNA

 

More information: Datasheet

 
Rat Gene 2.0 ST 

 

Sample requirement: 50 - 100ng FFPE total RNA

 

Datasheet

 

 
Rat Genome 230 2.0 

 

Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 

 
Rat Genome 230 2.0 

 

Sample Preparation: NuGEN Ovation V2 

Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

More information: Datasheet

 

 
RNA - Bioanalyzer Nano 

 

x ng/uL, submit 5 uL

 
RNA - Bioanalyzer Pico 

x ng/uL, submit 5 uL

 

 
Yeast Genome 2.0 


Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 

 
Yeast Genome 2.0 

 

Sample Preparation:NuGEN Ovation V2 

Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

Datasheet

 
Zebrafish Gene 1.0 ST 

 

Sample requirement: 300ng (minimum 100ng)

 

More information: Datasheet

 

 
Zebrafish Genome 

 

Sample requirement: 300ng (100ng minimum)

 

More information: Datasheet

 

 
Zebrafish Genome 

 

Sample Preparation: NuGEN Ovation V2

 Sample Requirement: 5ng-30ng (minimum requires very high quality RNA)

 More information: Datasheet

 

 

Fixed Cost Services


Search available services: View: by category alphabetically
3' Array Low Input (9)
3' Arrays (9)
FFPE Whole Transcript - Affymetrix SensationPlus (7)
FFPE Whole Transcript - NuGEN (9)
Other (1)
QC only (4)
SNP Arrays (4)
Whole Transcript Arrays (11)
Whole Transcript Arrays - Low Input (9)
miRNA Array (1)

Map



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Click on this map for specific directions to and from Logan Airport and the Mass Pike as well as a good place to eat while visiting MBCF.