MBCF: Genomics

Molecular Biology Core Facilities: Genomics Core Services

The Molecular Biology Core Facilities (MBCF) at Dana-Farber Cancer Institute (DFCI) offers a full suite of services for a wide variety of genomic next-generation sequencing (NGS) library preparation, sequencing and analysis applications including RNAseq, ChIPseq, ATACseq, CRISPR screening, whole genome methylation profiling, targeted resequencing, single-cell RNAseq, exome sequencing, and more. The genomics core technology platforms include Illumina NextSeq500s, MiSeqs, a MiniSeq, NovaSeq 6000, and NovaSeq X Plus. High throughput sample preparation is performed on Beckman Coulter Biomek FX and i7 systems. Low throughput precious samples are prepared by experienced technical staff. The MBCF writes and maintains standardized pipelines for routine NGS analysis such as RNAseq, ChIPseq, and CRISPR screening. Complex bioinformatics analysis such as integration of multi-omics datasets or specialized analyses can be performed by the experienced computational biology team.

Please visit our website for more detailed and updated information.

**IMPORTANT NOTICE**

NovaSeq 6000 to be retired October 2024
Due to broad uptake of the new Illumina NovaSeq X Plus sequencing platform, MBCF will no longer support sequencing on the older NovaSeq 6000 platform beginning October 1, 2024.The NovaSeq X plus is essentially better, faster, and cheaper than the NovaSeq 6000. As a result, almost all projects and services have already been transitioned to the NovaSeq X Plus platform. We do not anticipate that suspending NovaSeq 6000 services will have any significant impact for ongoing projects.

Please reach out to us if you have any questions or concerns.

Sample Drop-off Locations

Samples can be dropped off in the MBCF Fish room near 3rd floor elevator lobby of Smith, directly to our lab on the 4th floor of DFCI Fenway Campus, or shipped via FedEx.

Physical Street Address / Fedex Delivery:
Molecular Biology Core Facilities
Dana Farber Cancer Institute North Campus
21-27 Burlington Avenue 4th Floor
Boston, MA 02215
USA

US postal, UPS, Mailing Address:
Molecular Biology Core Facilities
Dana Farber Cancer Institute North Campus
450 Brookline Avenue, BA404
Boston, MA 02215
USA

Information for Users External to DFCI

Purchase Orders are to be made out to:

Dana Farber Cancer Institute

General Accounting, BP-425

450 Brookline Avenue

Boston, MA 02215-5450

Note to Partners, Harvard, Boston Children’s and Tufts researchers:

Partners Health Care (includes MGH, BWH, McLean, Spaulding, etc): choose vendor 0000008573
Harvard (includes HMS,Dental, Wyss, HSPH, etc.): choose vendor site: 01_PRIMARY
Tufts University: choose Supplier 0000000655
Boston Children’s Hospital: choose vendor 0000001886

If you are using a PO from an institution other than one under which you signed in, you must stop and contact the DFCI iLab staff at sc_billing@dfci.harvard.edu

Contacts

Name	Role	Phone	Email	Location
Zach Herbert	Core Director	617-632-3082	zherbert@mail.dfci.harvard.edu
Maura Berkeley	Lab Manager	617-632-3082	maura_berkeley@mail.dfci.harvard.edu

Initiate Service Requests

Name	Description	Price
Illumina MiSeq 150 v3 Sequencing	The MiSeq 150 v3 cycle kit contains 150 cycles of sequencing and can be broken up in many ways! Please be sure to indicate on the form what your read length requirements are. If you are unsure, don't hesitate to ask. Data Output: 22-25M reads Sample Requirement: >10nM in >10ul (if this is problem contact us) MBCF QC: Qubit, TapeStation, qPCR Turnaround time: 3-7 days	Inquire
Libraries for NovaSeq sequencing	We have moved all sequencing to the NovaSeq X Plus sequencer. You may purchase a full lane of sequencing returning 1.2B reads, or you can purchase reads in 100M increments. The service can be found under Illumina NovaSeq X Plus/LIBRARY submitted for 100M NovaSeq X reads or NovaSeq X 10B 300 cycle LANE Please inquire with the MBCF with any questions. Some uniquely dual-indexed libraries are eligible for sequencing on our NovaSeq 6000 sequencer, PE150. Please inquire with MBCF team. $250/100M reads (internal) $300/100M reads (external)	Inquire
PCR Library Indexing (Nextera)		Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Illumina MiSeq Next Gen Sequencing (1)
Illumina MiSeq 150 v3 Sequencing	The MiSeq 150 v3 cycle kit contains 150 cycles of sequencing and can be broken up in many ways! Please be sure to indicate on the form what your read length requirements are. If you are unsure, don't hesitate to ask. Data Output: 22-25M reads Sample Requirement: >10nM in >10ul (if this is problem contact us) MBCF QC: Qubit, TapeStation, qPCR Turnaround time: 3-7 days	Inquire
▼ ► Libraries for NovaSeq sequencing (1)
Name	Description	Price
Libraries for NovaSeq sequencing	We have moved all sequencing to the NovaSeq X Plus sequencer. You may purchase a full lane of sequencing returning 1.2B reads, or you can purchase reads in 100M increments. The service can be found under Illumina NovaSeq X Plus/LIBRARY submitted for 100M NovaSeq X reads or NovaSeq X 10B 300 cycle LANE Please inquire with the MBCF with any questions. Some uniquely dual-indexed libraries are eligible for sequencing on our NovaSeq 6000 sequencer, PE150. Please inquire with MBCF team. $250/100M reads (internal) $300/100M reads (external)	Inquire
▼ ► Archive (1)
Name	Description	Price
PCR Library Indexing (Nextera)		Inquire

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Click on this map for specific directions to and from Logan Airport and the Mass Pike as well as a good place to eat while visiting MBCF.