Molecular Pathology Core Laboratory (MPCL)

Overview of Services

The Molecular Pathology Core Laboratory group works to advance molecular pathology-based, cancer research. The group addresses significant scientific and clinical research needs, and has created a central hub where researchers can interact, collaborate, and utilize novel molecular pathology technologies to translate basic science discoveries into patient care applications for cancer. Our mission comprises comprehensive pathology annotation to encompass clinical, morphological and molecular characteristics, genetic discovery in primary human tumors, with limited material and in real time, novel biomarker identification and pre-clinical trials and transitioning assays to the clinical setting.

 

 

Molecular Pathology Core Lab Website

 

 

Inquiries? Please contact us at DFCI_Pathology@dfci.harvard.edu ;

 

 

Leadership

Kathleen H. Burns, M.D., Ph.D. 
Chair
Department of Oncologic Pathology

 

Pamela Groen
Associate Director 

 

Staff

 

Kristen Au

Senior Research Technician

  

Hours of Operation and Location

9:00am - 5:00pm                         450 Brookline Avenue, Jimmy Fund 2nd Floor, Boston, MA  02215

Monday - Friday                              

 

MPCL Service Pricing 

 

 

 

 

Service Name

Unit

DFCI

External

Corporate

Histology Service

 

 

 

 

Paraffin Tissue Processing & Embedding

Block

12.89

17.41

25.14

Paraffin Tissue Sectioning, first slide

Slide 

6.49

8.76

12.66

Tissue sectioning, additional slide

Slide 

3.67

4.95

7.16

Paraffin Tissue Sectioning, TMA

Slide 

10.00

13.50

19.50

Frozen Tissue Embedding

Block

20.00

27.00

39.00

Frozen Sectioning

Slide 

8.23

11.11

16.05

H&E Staining

Slide 

3.59

4.85

7.00

Bone Tissue Decalcification

sample

18.65

25.18

36.37

Cell Pellet Making, Processing & Embedding

each

85.00

114.75

165.75

Coring Block/ Scraping slide for DNA/RNA Extraction

Slide 

6.92

9.34

13.50

 

 

 

 

 

IHC Service (Primary Antibody not included)

 

 

 

 

Antibody Optimization

EA

300.00

405.00

585.00

IHC staining

Slide

36.96

49.90

72.07

IHC Dual Staining_chromogenic

Slide

63.69

85.98

124.20

Multi-plex FIHC Optimization

Panel

900.00

1215.00

1755.00

Multi-plex FIHC_Mono control

Slide

65.00

87.75

126.75

Multi-plex FIHC_3 markers

Slide 

95.00

128.25

185.25

Multi-plex FIHC_4 markers

Slide 

125.00

168.75

243.75

Multi-plex FIHC_5 markers

Slide 

155.00

209.25

302.25

Multi-plex FIHC_ 6 markers

Slide 

185.00

249.75

360.75

 

 

 

 

 

InSitu hybridization (Probe/KIT not included)

 

 

 

 

RNA ISH Optimization

Probe

580.00

783.00

1131.00

RNA ISH

Slide 

90.00

121.50

175.50

Multiplex Fluorecense RNA ISH

Slide 

125.00

168.75

243.75

TUNEL Staining

Slide

63.52

85.75

123.26

 

 

 

 

 

LCM

 

 

 

 

LCM (Core operated)

Round (Three Slides)

150.00

202.50

292.50

LCM Training (including supplies, reagents, sections and labor)

Round

800.00

1080.00

1560.00

Equpment Access (for Trained Users only)

 

 

 

 

LCM Equpment Access (users prepare all supplies)

Hour

50.00

67.50

97.50

Cryostat Equpment Access (users prepare all supplies)

Hour

25.00

33.75

48.75

 

 

 

 

 

GeoMX DSP Assays

 

 

 

 

GeoMx DSP Protein Assay_Biopsy/Surgical  (NOTE: Minimum 12 slides/project, will charge for 12 slides if less)

Slide

350.00

472.50

682.50

GeoMx DSP Protein Assay_TMA slide

Per 12 Cores

350.00

472.50

682.50

GeoMx DSP RNA Assay_Biopsy/Surgical tissue (NOTE: Minimum 4 slides/project, will charge for 4 slides if less)

Slide

380.00

513.00

741.00

GeoMx DSP RNA Assay__TMA slide

Per 12 Cores

350.00

472.50

682.50

GeoMx Cancer Transcriptome Atlas (CTA) Assay (NOTE: Minimum 4 slides/project, will charge for 4 slides if less)

Slide

330.00

445.50

643.50

GeoMx Whole Transcriptome Atlas (WTA) Assay (NOTE: Minimum 4 slides/project, will charge for 4 slides if less)

 Slide

350.00 

472.50 

682.50

Spectral Imaging 

 

 

 

 

Whole Slide Scan (10x) - Brightfield 

Slide

9.00

12.15

17.55

Whole Slide Image Acquisition (20x) - Brightfield 

Slide

18.00

24.00

31.00

TMA Whole Slide Scan (10X)- Brightfield

Slide

10.00

13.50

19.50

TMA Whole Slide Image Acquisition (20x) - Brightfield 

Slide

52.00

70.20

101.40

Whole Slide Image Acquisition (10x) - Fluorescence

Slide

16.00

21.60

31.20

Whole Slide Image Acquisition (20x) - Fluorescence_Biopsy

Slide

50.00

67.50

97.50

Whole Slide Image Acquisition (20x) - Fluorescence_Non-Biopsy

Slide

80.00

108.00

156.00

Image Analysis - Brightfield 

Slide

50.00

67.50

97.50

Image Analysis - Fluorescence 

Slide

138.00

186.30

269.10

 

 

 

 

 

Molecular Pathology 

 

 

 

 

DNA Extraction and Quant_for LCM or scraping samples

sample

65.00

75.00

90.00

RNA Extraction and Quant_for LCM or scraping samples

sample

65.00

75.00

90.00

DNA/RNA QC_Bioanalyzer

sample

20.00

27.00

39.00