RNA Interference (RNAi) Screening Facility

Summary

The RNAi Facility provides investigators with access to RNAi reagents and high throughput screening technology. Lentiviral-based RNAi constructs against the human and mouse genomes are provided by the RNAi Consortium of the Broad Institute (http://www.broad.mit.edu/genome_bio/trc/), and can be used to transduce a wide range of target cells, including primary and non-dividing cells. The Facility provides reagents, equipment, and technical expertise to design, optimize, perform and analyze cell-based, arrayed lentiviral RNAi screens. Additionally, lentiviral RNAi constructs against individual genes can be requested by members of the DF/HCC community.

Services

The RNAi Consortium lentiviral human and mouse shRNA libraries are available for genome-scale screening. Custom screening sets available upon request. High-throughput virus preparation services are also available.

Leadership

Director: William C. Hahn, M.D., Ph.D.

Major Equipment

 

  • BioTek Microfill microplate dispenser
  • Lentiviral BL2+ tissue culture facility
  • MDS Spectramax plate reader
  • Perkin-Elmer Envision multi-label plate reader
  • Perkin-Elmer Janus mini liquid handling robot

 

 

Location and hours of operation

Hours Location

Mon-Fri 9am-5pm

DANA 15, 44 Binney St., Boston, MA 02115

Information for Users External to DFCI

Purchase Orders are to be made out to:     

Dana Farber Cancer Institute                           

General Accounting, BP-425                             

450 Brookline Avenue                                        

Boston, MA 02215-5450

Links and Resources

    1. http://www.broadinstitute.org/rnai/public/
    2. http://www.dfhcc.harvard.edu/core-facilities/collaborative-rnai/

 

 

Contacts

Name Role Phone Email Location
Your Name Here

 

 
you@dfci.harvard.edu