The RNAi Facility provides investigators with access to RNAi reagents and high throughput screening technology. Lentiviral-based RNAi constructs against the human and mouse genomes are provided by the RNAi Consortium of the Broad Institute (http://www.broad.mit.edu/genome_bio/trc/), and can be used to transduce a wide range of target cells, including primary and non-dividing cells. The Facility provides reagents, equipment, and technical expertise to design, optimize, perform and analyze cell-based, arrayed lentiviral RNAi screens. Additionally, lentiviral RNAi constructs against individual genes can be requested by members of the DF/HCC community.
The RNAi Consortium lentiviral human and mouse shRNA libraries are available for genome-scale screening. Custom screening sets available upon request. High-throughput virus preparation services are also available.
Director: William C. Hahn, M.D., Ph.D.
Hours | Location |
Mon-Fri 9am-5pm |
DANA 15, 44 Binney St., Boston, MA 02115 |
Purchase Orders are to be made out to:
Dana Farber Cancer Institute
General Accounting, BP-425
450 Brookline Avenue
Boston, MA 02215-5450
Name | Role | Phone | Location | |
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Your Name Here |
you@dfci.harvard.edu
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