The Transgenic Targeting Core offers state-of-the-art instrumentation and staff with scientific and technical expertise in the area of transgenic and gene targeted mouse models.
The core assists first time and experienced investigators in the production and characterization of engineered mouse strains. The core provides the essential services, equipment, and reagents, as well as scientific and technical expertise to generate such models. Training is available on both the technical and conceptual levels.
Getting Started
Consultation: prior to any work commencing with Transgenic Targeting Core, you must schedule a consultation with the Core Director, James Horner. See below for contact info.
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BAC Transgene Microinjection Service (cost per pup)
Service Steps:
a . Experimental Design Consultation b . Transgene purification for injection c . Injection of construct into fertilized FVB eggs d . Transfer of injected eggs into foster mothers e . Monitoring of foster females and litters f . Toe and Tail docking
Inquire
Cloning of knock-in or knockout vector
Inquire
Design of targeting vector
Inquire
DNA isolation and screening of ES clones
Inquire
ES cell electroporation (additional set of 144 clones)
Cost for each additional set of 144 clones.
Note: A targeting vector and experimental design consultation is required before any targeting project is initiated.
Service Steps:
Expanstion & electroporation of proven germline ES cells
Picking and expansion of clones
Freezing clones
Clones grown in 48-well plates and released to investigator for DNA isolation and screening
Expansion and freezing of targeted clones
Cells derived from targeted clones are released for DNA isolation and Southern confirmation by the investigator
Inquire
ES cell electroporation (first 144 clones)
Cost for first set of 144 clones.
Note: A targeting vector and experimental design consultation is required before any targeting project is initiated.
Service Steps:
Expanstion & electroporation of proven germline ES cells
Picking and expansion of clones
Freezing clones
Clones grown in 48-well plates and released to investigator for DNA isolation and screening
Expansion and freezing of targeted clones
Cells derived from targeted clones are released for DNA isolation and Southern confirmation by the investigator
Inquire
ES Cell Microinjection Service (cost per offspring)
Service Steps:
a . Thawing and culture of targeted clone. b . Preparation of cells for injection. c . Microinjection of targeted clone into c57/B6 blastocysts. d . Transfer of injected blastocysts into fosters. e . Monitoring fosters and litters. f . Identification of chimeric offspring g . Chimeras released to investigator for germline test mating
Inquire
ES Clone Karyotyping
Inquire
Serological testing of chimera and release to investigator (provided by Charles River Labs)
Service provided by Charles River Labs - for service packages requiring this step, a separate PO will need to be cut to Charles River Labs.
Inquire
Transfer of injected balstocysts into fosters
Inquire
Transgene Microinjection Service FVB (cost per pup)
Service Steps:
a . Experimental Design Consultation b . Transgene purification for injection c . Injection of construct into fertilized FVB eggs d . Transfer of injected eggs into foster mothers e . Monitoring of foster females and litters f . Toe and Tail docking
BAC Transgene Microinjection Service (cost per pup)
Service Steps:
a . Experimental Design Consultation b . Transgene purification for injection c . Injection of construct into fertilized FVB eggs d . Transfer of injected eggs into foster mothers e . Monitoring of foster females and litters f . Toe and Tail docking
Inquire
ES Cell Microinjection Service (cost per offspring)
Service Steps:
a . Thawing and culture of targeted clone. b . Preparation of cells for injection. c . Microinjection of targeted clone into c57/B6 blastocysts. d . Transfer of injected blastocysts into fosters. e . Monitoring fosters and litters. f . Identification of chimeric offspring g . Chimeras released to investigator for germline test mating
Inquire
Transgene Microinjection Service FVB (cost per pup)
Service Steps:
a . Experimental Design Consultation b . Transgene purification for injection c . Injection of construct into fertilized FVB eggs d . Transfer of injected eggs into foster mothers e . Monitoring of foster females and litters f . Toe and Tail docking
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