MBCF: Proteomics

 

 MBCF Proteomics Core Services

• Peptide mass fingerprinting (PMF) or Protein fingerprinting(Next Day Results)
  
• Determine Post Translational Modifications (PTM) in complex samples
• Protein sequence determination
• Determine accurate intact mass of proteins and peptides
• Mass Spec Training for user based operation of MALDI instrument
• Amino Acid Analysis
• Elucidating chemical structures of molecules (i.e. peptides and other chemical compounds )
  

MBCF Proteomics Instrumentation:

• AB Sciex 4800 MALDI TOF-TOF
  
• Thermo Scientific LTQ Orbitrap XL
• Waters Nano-Acquity LC system
  
• Agilent 1200 LC for AAA

 

MBCF Proteomics Price List:

 

	Inside the Institute /CFAR	Outside the Institute
Intact mass analysis	$25.00	$50.00
tryptic digest and mass analysis by MALDI-TOF	$150.00 first sample, $50.00 additional samples	$300.00 first sample, $100.00 addtitional samples
tryptic digest and mass analysis by MALDI-TOF + LC-MS/MS	$200.00 first sample, $100.00 additional samples	$400.00 first sample, $200.00 addtitional samples
mass analysis of pre-digested sample by MALDI-TOF	$50.00	$100.00
Amino Acid Analysis	$35	$50
additional methods	call for quote	call for quote
1 hour user operated session	$50.00	$50.00
instrument operation tutorial (1-3 researchers)	$200.00	$200.00

 

Sample Drop-off Locations

 

Samples can be dropped off in the MBCF Fish room near 3rd floor elevator lobby of Smith, CLS building drop off on the 11th floor, or directly to our lab on the basement level 1, Longwood Center Building(LC B1052), or shipped via FedEx. 

 

 

 

Physical Street Address / Fedex Delivery:
Molecular Biology Core Facilities
Dana Farber Cancer Institute North Campus
21-27 Burlington Avenue 4th Floor
Boston, MA 02215
USA

 

US postal, UPS, Mailing Address:
Molecular Biology Core Facilities
Dana Farber Cancer Institute North Campus
450 Brookline Avenue, BA404
Boston, MA 02215
USA

 

 

Information for Users External to DFCI

Purchase Orders are to be made out to:

Dana Farber Cancer Institute

General Accounting, BP-425

450 Brookline Avenue

Boston, MA 02215-5450

 

Note to Partners, Harvard, Boston Children’s and Tufts researchers:

• Partners Health Care (includes MGH, BWH, McLean, Spaulding, etc): choose vendor 0000008573
• Harvard (includes HMS,Dental, Wyss, HSPH, etc.): choose vendor site: 01_PRIMARY
• Tufts University: choose Supplier 0000000655
• Boston Children’s Hospital: choose vendor 0000001886

 

 If you are using a PO from an institution other than one under which you signed in, you must stop and contact the DFCI iLab staff at sc_billing@dfci.harvard.edu

 

 

 

MBCF Amino Acid Analysis:

Amino Acid Analysis is a service that provided the researcher a quantative list of amino acids from their purifed protein or peptide sample.

Generally the sample is supplied in a non-amine containing buffer, and is then hydrolyzed in 6N HCl.  The hyrdolyzed mixture of amino acids is then derivatized with a fluorescent tag and seperated on an HPLC.  The result is a amino acids and their amounts that can be used for accurate quantitation in difficult to analyze samples.  Please call for more info.

 

 

 

 

Contacts

Name	Role	Phone	Email	Location
Jim Lee	Lab Manager	617-632-3082	j_lee@dfci.harvard.edu	LC B1 Rm 052
Paul Morrison	Director	617-632-3082	paul_morrison@dfci.harvard.edu

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